Human Beta Klotho Protein (KLB-HM101)

Product Overview

• Article Name: Human Beta Klotho Protein (KLB-HM101)
• Biozol Catalog Number: KAB-KLB-HM101
• Supplier Catalog Number: KLB-HM101
• Alternative Catalog Number: KAB-KLB-HM101-100UG, KAB-KLB-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: betaKlotho, beta-klotho, BKL, KLB, klotho beta like, Klotho beta, MGC142213,betaKlotho, beta-klotho, klotho beta like, Klotho beta

Product Description

Recombinant Human Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met30-Thr983.

Product Properties

• Molecular Weight: The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q86Z14
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Met30-Thr983
• Target: Beta Klotho

Images

Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).

Human Beta Klotho on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.

Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test).

Immobilized Human Beta Klotho at 2ug/ml (100ul/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test).

Immobilized Human FGF21, mFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA.