Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln22-Arg457.
Molecular Weight:
The protein has a predicted MW of 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Tag:
C-hFc (IgG1)
UniProt:
AAL36991.1
Buffer:
Lyophilized from 0.22µm filtered solution in 20mM Tris, 100mM glycine, 100mM L-arginine, 150mM NaCl (pH 7.5) / PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gln22-Arg457
Target:
LILRB2/CD85d/ILT4
Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag (Cat. HLG-HM41CT) with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200).
Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag (Cat. ANG-HM4L2) with an affinity constant of 0.14uM as determined in a SPR assay (Biacore T200).
Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 35.4ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human ANGPTL7, His Avi Tag (Cat. ANG-HM407) at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LILRB2, hFc Tag with the EC50 of 3.09ug/ml determined by ELISA.
Loaded Human LILRB2, hFc Tag on ProA-Biosensor can bind Human ANGPTL4, His Tag (Cat. ANG-HM1L4) with an affinity constant of 72.10 nM as determined in BLI assay (Gator Prime).
The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.
Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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