Non-biotinylated Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401)

Product Overview

• Article Name: Non-biotinylated Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401)
• Biozol Catalog Number: KAB-NEC-HM401
• Supplier Catalog Number: NEC-HM401
• Alternative Catalog Number: KAB-NEC-HM401-100UG, KAB-NEC-HM401-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD111, ED4, HIgRPVRR, HVEC, MGC142031, Nectin1, PRR, PRR1, PRR1MGC16207, PVRL1, PVRR1, SK-12, PVRL1, CLPED1, HIgR, OFC7, PVRR

Product Description

Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln31-Thr334.

Product Properties

• Molecular Weight: The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q15223
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Form: Lyophilized
• Sequence: Gln31-Thr334
• Target: Nectin-1/PVRL1/CD111

Images

Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Nectin-1 is greater than 90% as determined by SEC-HPLC.

Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).

Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 60.7ng/ml determined by ELISA (QC Test).

Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA.