Non-biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430)
Biozol Catalog Number:
KAB-NKP-HM430
Supplier Catalog Number:
NKP-HM430
Alternative Catalog Number:
KAB-NKP-HM430-100UG, KAB-NKP-HM430-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Alternative Names:
CD337, LY117, NCR3, NKp30, 1C7, MALS
Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138.
Molecular Weight:
The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-40 kDa based on Bis-Tris PAGE result.
Tag:
C-His-Avi
UniProt:
AAH52582
Buffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu19-Thr138
Target:
NKp30/NCR3/CD337
Human NKp30 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.
Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).
Immobilized Human NKp30, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Loaded Human B7-H6, hFc Tag on ProA-Biosensor, can bind Human NKp30, His Tag with an affinity constant of 0.51 µM as determined in BLI assay (Gator).
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