Human PD-1/PDCD1 Protein (PD1-HM101)

Product Overview

• Article Name: Human PD-1/PDCD1 Protein (PD1-HM101)
• Biozol Catalog Number: KAB-PD1-HM101
• Supplier Catalog Number: PD1-HM101
• Alternative Catalog Number: KAB-PD1-HM101-100UG, KAB-PD1-HM101-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: PDCD1, PD1, CD279, SLEB2, PD-1

Product Description

Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Gln167.

Product Properties

• Molecular Weight: The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q15116
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Leu25-Gln167
• Target: PD-1/PDCD1

Images

The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.

Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.

Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 2.20 uM as determined in SPR assay (Biacore T200).

Human PD-L2, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 0.21 uM as determined in SPR assay (Biacore T200).

Immobilized Human PD-1, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test).

Immobilized Human PD-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 77.8ng/ml determined by ELISA.

Loaded Biotinylated Human PD-L1, His Tag on Streptavidin-Biosensor can bind Human PD-1, His Tag with an affinity constant of 0.94 µM as determined in BLI assay (Gator Prime).

Loaded Human PD-L2, hFc Tag on ProA-Biosensor can bind Human PD-1, His Tag with an affinity constant of 1.17 µM as determined in BLI assay (Gator Prime).