Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc (TCC-HM101)
Biozol Catalog Number:
KAB-TCC-HM101
Supplier Catalog Number:
TCC-HM101
Alternative Catalog Number:
KAB-TCC-HM101-100UG
Manufacturer:
KactusBio
Category:
Sonstiges
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
NY-ESO-1 TCR&CD3
Recombinant Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the NY-ESO-1 TCR.It contains NY-ESO-1 specific TCR (1G4c113), CD3epsilon&CD3gamma, CD3epsilon&CD3delta, and CD3zeta dimers.
Molecular Weight:
The protein has a predicted MW of 194.8 kDa.
Tag:
C-His
Buffer:
Supplied as 0.22 µm filtered solution in PBS, 200mM L-arginine (pH 7.4). <RED>Notice: Not recommended for flow cytometry.</RED>
Expression System:
HEK293
Form:
Liquid
Sequence:
NY-ESO-1 specific TCR (1G4c113), CD3epsilon&CD3gamma, CD3epsilon&CD3delta, and CD3zeta dimers
Target:
NY-ESO-1 TCR&CD3
Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 18.3ng/ml determined by ELISA.
Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.1ug/ml determined by ELISA.
Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc captured on CM5 Chip via Streptavidin can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 0.78 nM as determined in SPR assay (Biacore T200).
Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.20 nM as determined in SPR assay (Biacore T200).
Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.89 nM as determined in BLI assay (Gator Prime).
Loaded Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 2.37 nM as determined in BLI assay (Gator Prime).
OKT3, mFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag with an affinity constant of 0.33 nM as determined in SPR assay (Biacore T200).
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