Human TIGIT Protein, Ultra Low Endotoxin (TIG-HM110-UL)

Product Overview

• Article Name: Human TIGIT Protein, Ultra Low Endotoxin (TIG-HM110-UL)
• Biozol Catalog Number: KAB-TIG-HM110-UL
• Supplier Catalog Number: TIG-HM110-UL
• Alternative Catalog Number: KAB-TIG-HM110-UL-100UG, KAB-TIG-HM110-UL-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: TIGIT, VSIG9, VSTM3, WUCAM

Product Description

Recombinant Human TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Pro141.

Product Properties

• Molecular Weight: The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
• Tag: C-His
• UniProt: Q495A1
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Met22-Pro141
• Target: TIGIT

Images

Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.

Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17uM as determined in a SPR assay (Biacore T200).

Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46ug/ml.

Immobilized Human TIGIT, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.

Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA (QC Test).