Human TRAIL/TNFSF10 Protein (active trimer) (TRL-HE001)

Product Overview

• Article Name: Human TRAIL/TNFSF10 Protein (active trimer) (TRL-HE001)
• Biozol Catalog Number: KAB-TRL-HE001
• Supplier Catalog Number: TRL-HE001
• Alternative Catalog Number: KAB-TRL-HE001-100UG, KAB-TRL-HE001-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: Apo-2L, TRAIL, CD253, TNFSF10, Apo-2 ligand, APO2L, TL2

Product Description

Recombinant Human TRAIL/TNFSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281.

Product Properties

• Molecular Weight: The protein has a predicted MW of 19.6 kDa same as Bis-Tris PAGE result.
• Tag: No Tag
• UniProt: NP_003801.1
• Buffer: Lyophilized from 0.22 µm filtered solution in 50mM Tris, 300mM NaCl (pH 7.2). Normally 8% mannitol is added as protectant before lyophilization.
• Expression System: E.coli
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Val114-Gly281
• Target: TRAIL/TNFSF10

Images

Human TRAIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC.

Human TRAIL R3, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).

Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).

Immobilized Human TRAIL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.

The purity of Human TRAIL is greater than 95% and the molecular weight of this protein is around 45-65 kDa as determined by SEC-MALS.