Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)

Catalog Number: KAB-TSP-CM101B
Article Name: Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B)
Biozol Catalog Number: KAB-TSP-CM101B
Supplier Catalog Number: TSP-CM101B
Alternative Catalog Number: KAB-TSP-CM101B-100UG, KAB-TSP-CM101B-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Monkey
Conjugation: Biotin
Alternative Names: thymic stromal lymphopoietin, TSLP
Recombinant Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr29-Gln159.
Molecular Weight: The protein has a predicted MW of 16.3 kDa. Due to furin cleavage site, the protein migrates to 10-13 kDa based on Bis-Tris PAGE result.
Tag: C-His
UniProt: A0A7N9CAT7
Buffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Purity: > 90% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form: Lyophilized
Sequence: Tyr29-Gln159
Target: TSLP
Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.
The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 13.40 nM as determined in BLI assay (Gator Prime).
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 1.12 nM as determined in BLI assay (Gator Prime).