Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly25-Lys231
Target:
TSLPR
Human TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
Immobilized Human TSLPR, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.
Immobilized Human TSLP (R127A, R130A), His Tag at 2ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP, His Tag with an affinity constant of 31.30 nM as determined in BLI assay (Gator Prime).
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