Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)

Product Overview

• Article Name: Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)
• Biozol Catalog Number: KAB-TSP-HM402B
• Supplier Catalog Number: TSP-HM402B
• Alternative Catalog Number: KAB-TSP-HM402B-100UG, KAB-TSP-HM402B-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin
• Alternative Names: Thymic stromal lymphopoietin, TSLP

Product Description

Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).

Product Properties

• Molecular Weight: The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: Q969D9
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Tyr29-Gln159(R127A,R130A)
• Target: TSLP

Images

Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.

Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator Prime).

Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 4.87 nM as determined in BLI assay (Gator Prime).