Non-biotinylated Human VEGF R1/FLT-1 Protein (VGF-HM4R1)

Product Overview

• Article Name: Non-biotinylated Human VEGF R1/FLT-1 Protein (VGF-HM4R1)
• Biozol Catalog Number: KAB-VGF-HM4R1
• Supplier Catalog Number: VGF-HM4R1
• Alternative Catalog Number: KAB-VGF-HM4R1-100UG, KAB-VGF-HM4R1-500UG
• Manufacturer: KactusBio
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: FLT, FLT1, Flt-1, FRT, VEGF R1, VEGFR1

Product Description

Recombinant Human VEGF R1/FLT-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Asn756.

Product Properties

• Molecular Weight: The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P17948
• Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Form: Lyophilized
• Sequence: Ser27-Asn756
• Target: VEGF R1/FLT-1

Images

Human VEGF R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 53.4ng/ml determined by ELISA.

Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 40.3ng/ml determined by ELISA (QC Test).

Biotinylated Human VEGF165, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).

Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF165, Llama IgG2b Fc Tag with the EC50 of 69.9ng/ml determined by ELISA.

The purity of Human VEGF R1 is greater than 95% as determined by SEC-HPLC.