TMB ONE

Catalog Number: KEM-4380H
Article Name: TMB ONE
Biozol Catalog Number: KEM-4380H
Supplier Catalog Number: 4380H
Alternative Catalog Number: KEM-4380H-100ML
Manufacturer: Kementec
Category: Sonstiges
Application: ELISA
ELISA HRP Substrate

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

 

TMB ONE characteristics are:

  • “The Gold Standard” used across the industry
  • Incredibly stable and robust
  • Aqueous based – no organic solvents (REACH Annex XVII compliant)
  • Low lot-to-lot variation

For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. KEM-4430*). The pink indicator disappears after addition of the stop solution.

Form: Ready-to-use (RTU)
Storage: 2-8⁰C in the dark
Application Notes: HRP in ELISA – The Gold Standard