HISTOPRIME Dulbeccos PBS, Powder, without Ca & Mg, without Phenol Red - for 5 L

Catalog Number: LIN-GBP1622LEA
Article Name: HISTOPRIME Dulbeccos PBS, Powder, without Ca & Mg, without Phenol Red - for 5 L
Biozol Catalog Number: LIN-GBP1622LEA
Supplier Catalog Number: GBP1622LEA
Alternative Catalog Number: LIN-GBP1622LEA,LIN-LIN-GBP1622LEA
Manufacturer: Linaris
Category: Zellen/Zellkultur
Application: Cell Culture
HISTOPRIME Dulbeccos PBS, Powder, without Ca and Mg, without Phenol Red

Formulation:

Components CasNo Formulation Concentration mg/L
Disodium Hydrogen Phosphate 7558-79-4 Na2HPO4 1150
Potassium Chloride 7447-40-7 KCl 200
Potassium Phosphate, Monobasic 7778-77-0 KH2PO4 200
Sodium Chloride 7647-14-5 NaCl 8000
TOTAL 9.550 mg/L

 

Amount: 47.75 g, for 5 L
Appearance: Off-white to creamy white, homogenous powder
Instruction: Powdered media and salts are very hygroscopic and have to be stored under dry conditions.
Use at: 9.55 g/L
pH: 7.5 ± 0.3
Osmolality: 285 mOsm/kg ± 10%
Storage and shelf life: Store at +20°C, dry and protected from light. Please refer to product label for expiration date.
Shipping conditions: Ambient

 

Usage

1) Measure out 90% of final required volume of water. Water temperature should be 15-20°C.
2) While gently stirring the water, add the powdered medium (9.55 g/L). Stir until dissolved. Do not heat.
3) Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2.
4) While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1 N HCl or 1 N NaOH is recommended.
5) Add additional water to bring the solution to final volume.
6) Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.
7) Aseptically dispense medium into sterile container.


This product is for in vitro research use only. It is not a medical device and it I not intended for diagnostic or therapeutic purposes

Form: Powder
Storage: 20 °C