Formulation:

Components	CasNo	Formulation	Concentration mg/L
Disodium Hydrogen Phosphate	7558-79-4	Na2HPO4	1150
Potassium Chloride	7447-40-7	KCl	200
Potassium Phosphate, Monobasic	7778-77-0	KH2PO4	200
Sodium Chloride	7647-14-5	NaCl	8000
TOTAL			9.550 mg/L

 

Amount: 47.75 g, for 5 L
Appearance: Off-white to creamy white, homogenous powder
Instruction: Powdered media and salts are very hygroscopic and have to be stored under dry conditions.
Use at: 9.55 g/L
pH: 7.5 ± 0.3
Osmolality: 285 mOsm/kg ± 10%
Storage and shelf life: Store at +20°C, dry and protected from light. Please refer to product label for expiration date.
Shipping conditions: Ambient

 

Usage

1) Measure out 90% of final required volume of water. Water temperature should be 15-20°C.
2) While gently stirring the water, add the powdered medium (9.55 g/L). Stir until dissolved. Do not heat.
3) Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2.
4) While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1 N HCl or 1 N NaOH is recommended.
5) Add additional water to bring the solution to final volume.
6) Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.
7) Aseptically dispense medium into sterile container.