IHC-plus(TM) ACTB / Beta Actin Antibody LS-B1040, Unconjugated, Polyclonal

Product Overview

• Article Name: IHC-plus(TM) ACTB / Beta Actin Antibody LS-B1040, Unconjugated, Polyclonal
• Biozol Catalog Number: LS-B1040-50
• Supplier Catalog Number: LS-B1040-50
• Alternative Catalog Number: LS-B1040-50
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A 16 AA synthetic peptide near the C-Terminus of human Beta-actin. The immunogen is located within the last 50 AA of Beta-actin.
• Conjugation: Unconjugated
• Alternative Names: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

Product Description

Beta Actin antibody LS-B1040 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) from human. It is reactive with human, mouse and rat. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 60
• Buffer: PBS containing 0.02% Sodium Azide
• Purity: Peptide Column Affinity Chromatography
• Form: PBS containing 0.02% Sodium Azide

Application Notes

• Application Dilute: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB
• Application Notes: Beta-actin antibody can be used for the detection of Beta-actin by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human

Images

Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10ug/ml.

Immunofluorescence of Beta-actin in HeLa cells with Beta-actin antibody at 20 ug/ml.

Western blot of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5,