IHC-plus(TM) Polyclonal Rabbit anti-Human BCKDHA / BCKDE1A Antibody (C-Terminus, IHC, IF, WB) LS-B12367

Product Overview

• Article Name: IHC-plus(TM) Polyclonal Rabbit anti-Human BCKDHA / BCKDE1A Antibody (C-Terminus, IHC, IF, WB) LS-B12367
• Biozol Catalog Number: LS-B12367-50
• Supplier Catalog Number: LS-B12367-50
• Alternative Catalog Number: LS-B12367-50
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: BCKDHA, BCKDH E1-alpha, BCKDE1A, MSU, MSUD1, OVD1A

Product Description

BCKDE1A antibody LS-B12367 is an unconjugated rabbit polyclonal antibody to human BCKDE1A (BCKDHA) (C-Terminus). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• NCBI: 593
• Buffer: 1X PBS, pH 7, 0.01% Thimerosal, 20% Glycerol, 1% BSA
• Purity: Immunoaffinity purified
• Form: 1X PBS, pH 7, 0.01% Thimerosal, 20% Glycerol, 1% BSA

Application Notes

• Application Dilute: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:3000)
• Application Notes: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:200 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other appli

Images

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE BCKDHA antibody diluted at 1:1000

Immunofluorescence of methanol-fixed HeLa, using BCKDHA antibody at 1:500 dilution.