IHC-plus(TM) Polyclonal Rabbit anti-Human SHMT / SHMT2 Antibody (IHC, IF, WB) LS-B14859

Product Overview

• Article Name: IHC-plus(TM) Polyclonal Rabbit anti-Human SHMT / SHMT2 Antibody (IHC, IF, WB) LS-B14859
• Biozol Catalog Number: LS-B14859-50
• Supplier Catalog Number: LS-B14859-50
• Alternative Catalog Number: LS-B14859-50
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). PSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLEL
• Conjugation: Unconjugated
• Alternative Names: SHMT2, GLY A+, GLYA, Hydroxymethyltransferase 2, Serine methylase, Serine aldolase, SHMT, Threonine aldolase, Serine hydroxymethylase

Product Description

SHMT2 antibody LS-B14859 is an unconjugated rabbit polyclonal antibody to SHMT2 (SHMT) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• NCBI: 6472
• Buffer: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
• Purity: Affinity purified
• Form: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Application Notes

• Application Dilute: IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:2000)
• Application Notes: The predicted MW is 53kDa/54kDa/55kDa, while the observed MW by Western blot was 52kDa.

Images

Immunofluorescence analysis of HepG2 cell using SHMT2 antibody. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded rat kidney tissue.

Western blot analysis of extracts of various cell lines, using SHMT2 antibody at 1:1000 dilution