IHC-plus(TM) Polyclonal Rabbit anti-Human LIG3 / DNA Ligase III Antibody (IHC, IF, WB) LS-B14872

Product Overview

• Article Name: IHC-plus(TM) Polyclonal Rabbit anti-Human LIG3 / DNA Ligase III Antibody (IHC, IF, WB) LS-B14872
• Biozol Catalog Number: LS-B14872-50
• Supplier Catalog Number: LS-B14872-50
• Alternative Catalog Number: LS-B14872-50
• Manufacturer: LifeSpan Biosciences
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human LIG3 (NP_039269.2). AGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSR
• Conjugation: Unconjugated
• Alternative Names: LIG3, DNA ligase III, Ligase II, DNA, ATP-dependent, LIG2, Ligase III, DNA, ATP-dependent, DNA ligase 3

Product Description

DNA Ligase III antibody LS-B14872 is an unconjugated rabbit polyclonal antibody to human DNA Ligase III (LIG3). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.53 mg/ml
• NCBI: 3980
• Buffer: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
• Purity: Affinity purified
• Form: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Application Notes

• Application Dilute: IF (1:50 - 1:100), IHC (1:50 - 1:200), IHC-P (1:200), IP (1:50 - 1:200), WB (1:500 - 1:2000)
• Application Notes: The predicted MW is 95kDa/102kDa/106kDa/112kDa, while the observed MW by Western blot was 113kDa.

Images

Western blot analysis of extracts of various cell lines, using LIG3 antibody(1:1000).

Immunofluorescence analysis of MCF7 cell using LIG3 antibody. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded rat heart.