Polyclonal Rabbit anti-Human ARAF / ARAF1 / A-RAF Antibody (aa371-554, IHC, WB) LS-C185401

Catalog Number: LS-C185401-100
Article Name: Polyclonal Rabbit anti-Human ARAF / ARAF1 / A-RAF Antibody (aa371-554, IHC, WB) LS-C185401
Biozol Catalog Number: LS-C185401-100
Supplier Catalog Number: LS-C185401-100
Alternative Catalog Number: LS-C185401-100
Manufacturer: LifeSpan Biosciences
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, IHC-P, WB
Species Reactivity: Human
Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: ARAF, A RAF, A-RAF, A-raf-1, PKS, PKS2, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Ras-binding protein DA-Raf, Oncogene ARAF1, ARAF1, Proto-oncogene Pks, RAFA1
A-RAF antibody LS-C185401 is an unconjugated rabbit polyclonal antibody to human A-RAF (ARAF / ARAF1) (aa371-554). Validated for ICC, IHC and WB.
Clonality: Polyclonal
Concentration: 0.85 mg/ml
NCBI: 369
Buffer: 0.1 M Tris, pH 7, 0.1 M Glycine, 10% Glycerol, 0.01% Thimerosal
Purity: Immunoaffinity purified
Form: 0.1 M Tris, pH 7, 0.1 M Glycine, 10% Glycerol, 0.01% Thimerosal
Application Dilute: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)
Application Notes: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Immunofluorescence of paraformaldehyde-fixed A431 using ARAF antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. ARAF / A RAF antibody
IHC of paraffin-embedded Cal27 xenograft using ARAF antibody at 1:500 dilution.