Synthetic peptide (KLH-coupled) corresponding to amino acids at the N-Terminus of human NEDD8 protein.
Conjugation:
Unconjugated
Alternative Names:
NEDD8, NEDD-8, Neddylin, Ubiquitin-like protein Nedd8
NEDD8 antibody LS-C37486 is an unconjugated rabbit polyclonal antibody to NEDD8 (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
Purity:
Immunoaffinity purified
Form:
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
Application Dilute:
Flo (1:200), IF (1:200), IHC, IHC-P, IP (1:25), WB (1:1000)
Application Notes:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Flow Cytometry. Western Blot 1:1000. Immunoprecipitation 1:25. Immunofluorescence (IF-IC) 1:200. Flow Cytometry: 1:200.
Western blot of NEDD8
IHC of paraffin-embedded human colon carcinoma
Flow cytometry of untreated HeLa cells
Immunofluorescence of paraformaldehyde-fixed HeLa cells
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