Anti-LC3 (Human) pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Anti-LC3 (Human) pAb, Rabbit, Polyclonal
• Biozol Catalog Number: MBL-PM036
• Supplier Catalog Number: PM036
• Alternative Catalog Number: MBL-PM036
• Manufacturer: MBL
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IHC, IP, WB
• Species Reactivity: Hamster, Human, Mouse, Rat
• Immunogen: Recombinant human LC3 (MAP1LC3B :1-120 a.a.)
• Alternative Names: autophagy-related protein LC3 A, autophagy-related protein LC3 B, autophagy-related ubiquitin-like modifier LC3 A, autophagy-related ubiquitin-like modifier LC3 B, LC3A, LC3B, LC3-I, LC3-II, MAP1 light chain 3-like protein 1, MAP1 light chain 3-like protein 2, MAP1A/1B light chain 3 A, MAP1A/1B light chain 3 B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B light chain 3 B, Map1lc3, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A, microtubule-associated proteins 1A/1B light chain 3B, Mpl3, zbs559

Product Description

Anti-LC3 (Human) pAb, IgG, Rabbit, Polyclonal, Rb

Additional Information

LC3, a mammalian homologue of yeast ATG8, is a soluble protein that is distributed ubiquitously in mammalian tissues and cultured cells. LC3 has been studied most extensively and is frequently used as an autophagy marker in mammals. 

This LC3 antibody is validated for multiple applications (WB, IHC, ICC and IP) and has over 85 citations, including in research papers with more than 1,000 citations (Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)). This is a polyclonal antibody of 100 ul that is raised in rabbit and is reactive with human, hamster, mouse, rat.

Product Properties

• Clonality: Polyclonal
• Isotype: IgG
• NCBI: 8
• Buffer: 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Source: This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
• Form: 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Storage: -20°C
• Formula: 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Target: LC3
• Notes: For Research Use Only. Not for use in diagnostic procedures.

Application Notes

• Application Dilute: WB: 1:1000 (chemiluminescence detection system). IP: 2 µL/300 µL of cell extract from 1x107 cells. FCM: 1:200 (final concentration). ICC: 1:500-1:1000. IHC: 1:1000-1:2000 (heat treatment required for paraffin)

Images

Anti-LC3 (Human) pAb

PM036

PM036

PM036