TransIT-LT1 Transfection Reagent

Product Overview

• Article Name: TransIT-LT1 Transfection Reagent
• Biozol Catalog Number: MIR-2304
• Supplier Catalog Number: MIR-2304
• Alternative Catalog Number: MIR-2304,MIR2304,MIR 2304
• Manufacturer: Mirus Bio
• Category: Molekularbiologie

Product Description

The TransIT-LT1 (Low Toxicity) Transfection Reagent is a broad spectrum, high efficiency DNA transfection reagent that is easy-to-use and exhibits minimal cellular toxicity. This reagent is a proprietary forumation of polyamines and cationic lipids that effiently transfects cells in the presence of serum. Broad Spectrum DNA Delivery - Utilize one transfection reagent and protocol for a variety of cells. Low Cellular Toxicity - Maintain cell density and reduce experimental bias. Deliver Single or Multiple Plasmids - Suitable for many applications such as gene expression, shRNA expression, virus production and promoter analysis

Images

The TransIT-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT-LT1 Transfection Reagent (Mirus Bio), cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection by flow cytometry.

TransIT-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. Hep G2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturers recommended reagent-to-DNA ratio indicated beneath each bar. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24-hours post-transfection. LDH levels are reported as percent cytotoxicity compared to cells alone. Experiments were performed as per industry accepted testing protocols. FuGENE is a registered trademark of Fugent LLC.