Product Description
Bacillus subtilis Positive Control Vectors: As a non-pathogenic, Gram-positive strain, Bacillus subtilis is well-known for its efficient production of recombinant proteins. Bacillus subtilis presents a highly advantageous host for biotechnological applications. It requires only simple culture conditions and exhibits a rapid growth cycle. While it shares these operational advantages with Escherichia coli, Bacillus subtilis is distinguished by its lack of pyrogenic lipopolysaccharides and its highly efficient protein secretion system. The following positive control vectors validated for recombinant protein production in Bacillus subtilis are available: • pHT01-bgaB (MOB-PBS001C) - for intracellular production of β-galactosidase after addition of IPTG • pHT43-amyQ (MOB-PBS002C) - for expression and secretion of α-amylase after addition of IPTG • pHT10-gfp+ (MOB-PBS005C) - for intracellular production of GFP+ after addition of IPTG • pHT2071 (MOB-PBS008C) - for constitutive intracellular production of β-galactosidase
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