Product Description
Mobicol Spin G-50 Columns for Nucleic Acid Purification. Mobicol Spin G-50 columns pre-packed with Sephadex® G-50 resin remove small molecules from DNA/RNA, such as salt ions, and are therefore most suitable for buffer exchange (even from dsDNA fragments as small as 20 bp). They can also be used to remove fluorescent dye dideoxy terminators (e.g., Cy3/Cy5 nucleotides) from cycle sequencing reactions or unincorporated labeled nucleotides (dye- or radiolabeled dNTPs or ddNTPs) from DNA labeling reactions (e.g., PCR probe labeling, Nick Translation, or DNA end-labeling). The purified DNA qualifies for downstream applications like FISH (fluorescence in situ hybridization) or Southern/Northern blotting. The removal of unincorporated labeled nucleotides is a precondition for determining the DNA labeling rate. For good recovery rates, labeled DNA fragments must be at least 20 bp in length. Applications: • Buffer exchange • Nucleotide removal • Removal of unincorporated dyes or dye terminators. (Sephadex® is a registered trademark of Cytiva)
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