Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to
Clonality:
Monoclonal
Concentration:
Concentration after reconstitution: 1.00 mg/ml as determined by A280 nm (A280 nm = 1.47 corresponds to 1 mg/ml antibody).
Clone Designation:
J2
Isotype:
IgG2a kappa
Buffer:
Mouse monoclonal antibody J2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurri
Source:
Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freunds adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate t
Purity:
Gel electrophoretically pure IgG antibody.
Formula:
The lyophilised sample should be reconstituted with 500 µl sterile distilled water. The mAb will then be in PBS without any stabilisers at a concentration of 1 mgr/ml. As a result of the lyophilisation procedure, the reconstituted antibody may contain sm
Application Notes:
Mouse monoclonal antibody J2 can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry (see references). The optimum working dilution of the antibody for any specific application shoul
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