KIF14 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: KIF14 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-13-549
• Supplier Catalog Number: 13-549
• Alternative Catalog Number: PRS-13-549-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14 (NP_055690.1).
• Conjugation: Unconjugated
• Alternative Names: MCPH2MKS12, kinesin-like protein KIF14, epididymis secretory sperm binding protein

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 190kDa
• NCBI: 9928
• UniProt: Q15058
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Images

Western blot analysis of extracts of various cell lines, using KIF14 antibody (13-549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat ovary using KIF14 antibody (13-549) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using KIF14 antibody (13-549) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF14 antibody (13-549) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using KIF14 antibody (13-549) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using KIF14 antibody (13-549) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using KIF14 antibody (13-549) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L929 cells using KIF14 Polyclonal Antibody (13-549) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using KIF14 Polyclonal Antibody (13-549) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.