ACE2 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	ACE2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3215
Supplier Catalog Number:	3215
Alternative Catalog Number:	PRS-3215-0.02,PRS-3215-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-ACE2 antibody (3215) was raised against a peptide corresponding to 17 amino acids near the center of human ACE2. The immunogen is located within amino acids 180 - 230 of ACE2.
Conjugation:	Unconjugated
Alternative Names:	ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation)
NCBI:	59272
UniProt:	Q9BYF1
Buffer:	ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-2 µg/mL, IHC: 2 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human and rat samples. All other applications and species not yet tested.

	Figure 6 Immunofluorescence Validation of ACE2 in Human Testis Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with 3215 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 7 Immunofluorescence Validation of ACE2 in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with 3215 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 8 Immunofluorescence Validation of ACE2 in Rat Lung Tissue Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with 3215 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 9 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with 3215 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.
	Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (3215) at 2 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 &956,g of lysates per lane.Antibodies: ACE2, 3215 (2 &956,g/mL), ACE2, 3217 (2 &956,g/mL), ACE2, 3227 (2 &956,g/mL) and beta-actin 3779 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation in Human TissuesLoading: 15 &956,g of lysates per lane.Antibodies: ACE2, 3215 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Mouse TissuesLoading: 15 &956,g of lysates per lane.Antibodies: ACE2, 3215 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Rat Skin TissueLoading: 15 &956,g of lysates per lane.Antibodies: ACE2, 3215 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.