CTLA4 Antibody [8A1], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: CTLA4 Antibody [8A1], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: PRS-RF16013
• Supplier Catalog Number: RF16013
• Alternative Catalog Number: PRS-RF16013-0.02,PRS-RF16013-0.1
• Manufacturer: ProSci
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
• Conjugation: Unconjugated
• Alternative Names: CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [8A1]
• Molecular Weight: Predicted: 25 kDa Observed: 32 kDa
• Isotype: IgG1
• NCBI: 1493
• UniProt: P16410
• Buffer: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Images

Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 &956,g/mL. Red: CTLA4 Antibody [8A1] (RF16013) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 &956,g/mL.

Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 &956,g/mL. Red: CTLA4 Antibody [8A1] (RF16013) Blue: DAPI staining

Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 &956,g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 &956,g/mL.

Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 &956,g/mL.

Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 &956,g/mL.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations.

A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, RF16013, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.

Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1 &956,g/mL.