AHSP Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: AHSP Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-100-401-E79
• Supplier Catalog Number: 100-401-E79
• Alternative Catalog Number: ROC-100-401-E79
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-AHSP antibody, Alpha-hemoglobin-stabilizing protein (AHSP), Erythroid differentiation-related factor, Erythroid-associated factor, EDRF, ERAF

Product Properties

• Clonality: Polyclonal
• Concentration: 115 mg/mL by Refractometry
• NCBI: 51327
• UniProt: Q9NZD4
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:8000, WB: 1:500-1:1000
• Application Notes: Anti-AHSP Antibody has been tested in western blot and immunohistochemistry and is suitable for immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by

Images

Immunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A.) Normal bone marrow, H&E. B.) CD235a stains both nucleated EPs and mature, anucleate RBCs. C.) AHSP stains nucleated EPs, but not mature, anucleate RBCs. D.) CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4C. Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max(TM) - fully automated for IHC.

Immunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A.) Acute erythroleukemia, H&E. B.) CD235a stains erythroid blasts and mature, anucleate RBCs. C.) AHSP stains erythroid blasts. D.) CD71 stains erythroid blasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4C. Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max(TM) - fully automated for IHC.

Immunohistochemistry of Rabbit anti-AHSP antibody. CD71 stains myeloid blasts in acute myeloid leukemia, whereas AHSP does not. AHSP stains residual EPs and not myeloid blasts in acute myeloid leukemia with minimal differentiation. (A), whereas CD71 stains both myeloid blasts and EPs (B). AHSP does not stain myeloid blasts in acute myelomonocytic leukemia (D), whereas CD71 does (E). C and F are corresponding H&Es, respectively. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max(TM) - fully automated for IHC.

Immunohistochemistry of Rabbit anti-AHSP antibody. AHSP and CD71 stain megakaryocytes in primary myelofibrosis. Tissue: A.) Primary myelofibrosis, H&E. B.) CD235a stains both nucleated EPs and mature, anucleate RBCs. AHSP C.) and CD71. D.) variably stain megakaryocytes and also stain nucleated EPs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max(TM) - fully automated for IHC.

Immunohistochemistry of Rabbit anti-AHSP antibody. AHSP (A) stains residual EPs and not lymphoma cells in DLBCL, whereas CD71 (B) stains both lymphoma cells and EPs. C. Corresponding H&E. AHSP (D) does not metastatic carcinoma, whereas CD71 (E) does. F. Corresponding H&E. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max(TM) - fully automated for IHC.

Immunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A.) Giant pronormoblasts are evident in parvoviral infection, H&E. B.) CD235a does not stain giant pronormoblasts. C.) AHSP and D.) CD71 stain giant pronormoblasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antig