Rhodamine Antibody, IgG1, Clone: [11H10], Mouse, Monoclonal

Product Overview

• Article Name: Rhodamine Antibody, IgG1, Clone: [11H10], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-246
• Supplier Catalog Number: 200-301-246
• Alternative Catalog Number: ROC-200-301-246
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: DOT, ELISA
• Immunogen: Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.
• Conjugation: Unconjugated
• Alternative Names: Mouse Anti-Rhodamine Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1.115 mg/mL by UV absorbance at 280 nm
• Clone Designation: [11H10]
• Isotype: IgG1
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:30,000, IHC: 1:1,000 - 1:10,000, WB: 1:1,000 - 1:10,000
• Application Notes: This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.

Images

Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin. Row 2 - Streptavidin. Load: Column 1 - 100ng. Column 2 - 33.3ng. Column 3 - 11.1ng. Column 4 - 3.70ng. Column 5 - 1.23ng. Primary antibody: Mouse anti-Rhodamine at 1:1,000 for 60 min at RT. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 1 HR at RT.

Rhodamine core structure. TRITC (tetramethylrhodamine-5-(and-6)-isothiocyanate) is the base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group (-N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.