DYKDDDDK Tag (Anti-FLAG) Antibody, IgG2a, Clone: [29E4.G7], Mouse, Monoclonal
Biozol Catalog Number:
ROC-200-301-383
Supplier Catalog Number:
200-301-383
Alternative Catalog Number:
ROC-200-301-383
Manufacturer:
Rockland Immunochemicals
Host:
Mouse
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG(TM) epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
Conjugation:
Unconjugated
Alternative Names:
mouse anti-FLAG(TM) tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Clonality:
Monoclonal
Concentration:
1.026 mg/mL by UV absorbance at 280 nm
Clone Designation:
[29E4.G7]
Isotype:
IgG2a
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:150,000 - 1:250,000, Flow Cytometry: User Optimized, IHC: User Optimized, WB: 1:2,000 - 1:20,000
Application Notes:
Anti-FLAG has been tested by ELISA and western blot. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most oft
Monoclonal Antibody to detect FLAG(TM) conjugated proteins detects both C terminal linked and N terminal linked FLAG(TM) tagged recombinant proteins by western blot.
Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 µL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab-anti-FLAG(TM) antibody. Personal Communication: A. Morrison and B. Kloss, NYCOMPS, New York, NY.
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