Anti-Aldh1l1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Aldh1l1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugation:
Unconjugated
Alternative Names:
Goat Anti-Aldh1l1 Antibody, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1, Fthfd
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:50,000, IHC: 1:200, IF Microscopy: 15 µg/mL, WB: 1:1000
Application Notes:
Anti-Aldh1l1 Antibody has been tested in WB, IHC, and IF. Expect a band at ~98.7kDa in western blot using appropriate lysates. Positive control used: NIH3T3 and Ms Liver for WB, Ms Liver and Kidney for IHC, and NIH3T3 for IF.
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Tissue: NIH3T3. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary antibody: Aldh1l1 antibody at 15µg/mL overnight at 2-8C. Secondary antibody: Donkey Anti-Goat IgG DyLight(TM) 488 Conjugated Preadsorbed (p/n 605-741-125) at 5µg/mL for 1 h at RT. Localization: cytoplasm. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Tissue: NIH3T3. Fixation: 100% MeOH. Permeabilization: 0.3%Triton X-100. Primary antibody: Aldh1l1 antibody at 15µg/mL overnight at 2-8C. Secondary antibody: Donkey Anti-Goat IgG DyLight(TM) 488 Conjugated Preadsorbed (p/n 605-741-125) at 5µg/mL for 1 h at RT. Localization: cytoplasm. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.
Immunohistochemistry of Goat Anti-Aldh1l1 antibody. Tissue: mouse Kidney tissue. Antigen retrieval: heat induced (HIER) using Citrate Buffer for 20min. Primary antibody: Aldh1l1 antibody at 1:200 for 30min at RT. Secondary Antibody: Donkey Anti-Goat HRP-IgG 4µl/mL for 20min at RT. Staining: DAB. Counter Stain: Hematoxylin. Localization: Aldh1l1 showed selective staining of renal tubular epithelial cells in mouse kidney. The specificity of the staining is cytoplasmic and membranous in tubules not in glomeruli.
Immunohistochemistry of Goat Anti-Aldh1l1 antibody. Tissue: mouse Liver tissue. Antigen retrieval: heat induced (HIER) using Citrate Buffer for 20min. Primary antibody: Aldh1l1 antibody at 1:200 for 30min at RT. Secondary Antibody: Donkey Anti-Goat HRP-IgG 4µl/mL for 20min at RT. Staining: DAB. Counter Stain: Hematoxylin. Localization: Aldh1I1 showed selective cytoplasmic staining in mouse liver of hepatocytes. The specificity of the staining is weak in bile duct cells and strong membranous and cytoplasmic in hepatocytes.
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Cells: HepG2 cells. Fixation: 100% MeOH. Permeabilization: 0.3%Triton X-100. Primary antibody: Anti-Aldh1l1 antibody at 15µg/mL overnight at 2-8C. Secondary antibody: Donkey Anti-Goat IgG DyLight(TM) 488 Conjugated Preadsorbed (p/n 605-741-125) at 5µg/mL for 1 h at RT. Localization: cytoplasm. Images were deconvoluted. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.
Western Blot of Goat Anti-Aldh1l1 Antibody. Lane 1: Opal Pre-stained MW ladder (p/n MB-210-0500). Lane 2: ALDH1L1 recombinant human protein. Lane 3: HEK293T Lysate (p/n W09-001-GX5). Lane 4: NIH3T3 Lysate (p/n W10-000-358). Lane 5: Mouse liver (normal tissue) Lysate (p/n W10-000-T020). Primary antibody: Aldh1l1 antibody at 1:1000 for overnight at 4C. Secondary antibody: F(ab)2 Donkey anti-Goat HRP secondary antibody at 1:40,000 for 1 hr at RT. Block: BlockOut (p/n MB-073) 30 min at RT. Predicted/Observed size: ~98.8kDa.
Western Blot of Goat Anti-Aldh1l1 Antibody. Lane 1: Opal Pre-stained MW ladder (p/n MB-210-0500). Lane 2: NIH3T3 Lysate (p/n W10-000-358). Lane 3: Mouse liver Lysate (p/n W10-000-T020). Lane 4: ALDH1L1 HEK293T overexpressed Lysate. Primary antibody: Aldh1l1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Donkey anti-Goat HRP secondary antibody (p/n 605-703-125) at 1:40,000 for 1 hr at RT. Block: BlockOut (p/n MB-073) 30 min at RT. Predicted/Observed size: ~106kDa overexpressed, ~98.8kDa/34kDa endogenous.
Western Blot of Goat Anti-Aldh1l1 Antibody. Lane 1: Opal Pre-stained MW ladder (p/n MB-210-0500). Lane 2: NIH3T3 Lysate (p/n W10-000-358). Primary antibody: Aldh1l1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Donkey anti-Goat HRP secondary antibody (p/n 605-703-125) at 1:40,000 for 1 hr at RT. Block: BlockOut (p/n MB-073) 30 min at RT. Predicted/Observed size: ~98.8kDa/34kDa endogenous.
* VAT and and shipping costs not included. Errors and price changes excepted
