TRPV4 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: TRPV4 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-FQ7
• Supplier Catalog Number: 600-401-FQ7
• Alternative Catalog Number: ROC-600-401-FQ7
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Anti-TRPV4 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the internal region of human TRPV4.
• Conjugation: Unconjugated
• Alternative Names: TRPV4 Antibody, SMAL, VRL2, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1, Transient receptor potential cation channel subfamily V member 4, Osm-9-like TRP channel 4, TrpV4

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 59341
• UniProt: Q9HBA0
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1-2 µg/mL
• Application Notes: Anti-TRPV4 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 98 kDa in Western Blots of specifi

Images

Immunofluorescence of TRPV4. Tissue: Human Kidney. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: TRPV4 at 5 µg/mL. Secondary: goat anti-rabbit IgG secondary antibody at 1:500 dilution (red) and DAPI staining (blue).

Immunohistochemistry of TRPV4. Tissue: rat kidney tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-TRPV4 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.

Western Blot of TRPV4.Load: 15µg of human cell lysates per lane. Primary Antibody: TRPV4 at 0.5 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry of TRPV4. Tissue: mouse kidney tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-TRPV4 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.

Western Blot of TRPV4.Load: 15µg of human tissue lysates per lane. Primary Antibody: TRPV4 at 0.5 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry of TRPV4. Tissue: human liver tissue. Fixation: paraffin-embedded, fixed with formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-TRPV4 antibody at 2 µg/ml overnight at 4C. Secondary: goat anti-rabbit IgG H&L (HRP) at 1:250. Counter stained with Hematoxylin.

Western Blot of TRPV4.Load: 15µg of mouse tissue lysates per lane. Primary Antibody: TRPV4 at 0.5 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot of TRPV4.Load: 15µg of rat tissue lysates per lane. Primary Antibody: TRPV4 at 0.5 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.