0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
Flow Cytometry: 1:500-1:2,500, IF Microscopy: 1:500 - 1:2,500
Application Notes:
F(ab)2 Anti-Rabbit IgG F(c) Fluorescein Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background l
Dot Blot results of Goat F(ab)2 Anti-Rabbit IgG F(c) Antibody Fluorescein Conjugate. Dots are Rabbit IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat F(ab)2 Anti-Rabbit IgG F(c) Antibody FITC at 1ug/mL in MB-070 1hr RT. Imaged with BioRad ChemiDoc, Fluorescein filter.
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