Goat Fab anti-Rat IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Goat Fab anti-Rat IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-812-1102
• Supplier Catalog Number: 812-1102
• Alternative Catalog Number: ROC-812-1102
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Goat Fab Anti-Rat IgG Antibody, Goat Fab Fragment Anti-Rat IgG Antibody
• Format: Fab
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Fab Anti-Rat IgG (H&L) Antibody has been tested by ELISA and dot blot and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificit

Images

Dot Blot of Fab Goat Anti-Rat IgG Antibody. Rat IgG (p/n 012-0102) (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Fab Goat Anti-Rat IgG at 1.0µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 605-703-125) at 1:40,000 for 30 mins at RT. Block: BlockOut buffer (p/n MB-073) for 30 mins at RT. Exposure: 5 sec.