SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified)

Article Name:	SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified)
Biozol Catalog Number:	SIN-40591-V08H
Supplier Catalog Number:	40591-V08H
Alternative Catalog Number:	SIN-40591-V08H-100
Manufacturer:	Sino Biological
Category:	Proteine/Peptide
Species Reactivity:	Virus
Alternative Names:	SARS-COV, COVID-19, COVID19, CORONA, COV2, Spike, NCOV, SARS-COV-2, COVID, COV-2

Purity

> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (40591-V08H), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (Cat: 40591-V08H) is 200-600 ng/mL.
2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.

Protein Construction

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus.

Accession#

YP_009724390.1

Expressed Host

HEK293 Cells

Species

2019-nCoV

Predicted N Terminal

Val16

Molecule Mass

The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Tag:	HIS Tag
Buffer:	Lyophilized from sterile PBS, pH 7.4.
Purity:	> 90 % as determined by SDS-PAGE.


	Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (S1, His tag) (40591-V08H), the EC50 of 2019-nCoV Spike Protein (S1, His tag) (40591-V08H) is 200-400 ng/mL.

	Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.