SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified)
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Article Name: | SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified) |
Biozol Catalog Number: | SIN-40591-V08H |
Supplier Catalog Number: | 40591-V08H |
Alternative Catalog Number: | SIN-40591-V08H-100 |
Manufacturer: | Sino Biological |
Category: | Proteine/Peptide |
Species Reactivity: | Virus |
Alternative Names: | SARS-COV, COVID-19, COVID19, CORONA, COV2, Spike, NCOV, SARS-COV-2, COVID, COV-2 |
Purity > 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. Endotoxin < 1.0 EU per μg protein as determined by the LAL method. Activity 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (40591-V08H), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (Cat: 40591-V08H) is 200-600 ng/mL. Protein Construction A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus. Accession# Expressed Host HEK293 Cells Species 2019-nCoV Predicted N Terminal Val16 Molecule Mass The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa. Formulation Lyophilized from sterile PBS, pH 7.4. Please refer to the specific buffer information in the hard copy of CoA. Shipping In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Stability & Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
Tag: | HIS Tag |
Buffer: | Lyophilized from sterile PBS, pH 7.4. |
Purity: | > 90 % as determined by SDS-PAGE. |