Product Description
Streptokinase G is not a protease, but it activates plasminogen by complexing with it. As a potential virulence factor, it is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells. Streptokinase G activates plasminogen to initiate fibrinolysis, making it a critical tool for cardiovascular research focused on thrombolytic mechanisms. This tag-free recombinant form, covering residues 27-440 of the mature protein, ensures the plasminogen-binding interface remains unobstructed-a prerequisite for accurate kinetic parameter analysis (Km, Vmax, kcat) and inhibitor screening with reliable IC50 determination. The specific activity of 8*104 IU/mg, validated by fibrin lysis in an agarose plate assay, confirms full biological function and supports use as a quantitative positive control in enzyme-linked fibrinolytic assays or substrate specificity profiling experiments. Purity exceeding 97% by SDS-PAGE combined with endotoxin levels below 1.0 EU/µg satisfies the criteria typical for drug candidate evaluation studies and provides a suitable basis for structural investigations such as crystallography or cryo-EM. Recombinant protein corresponding to aa27-440 from Streptococcus sp Streptokinase G, expressed in E. coli. Swiss/Uniprot: P10519, Streptococcus sp. (strain 19909) Molecular Weight: ~47.3kD Appearance: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O, 0.1% BSA to a concentration of 0.1-1mg/ml. Purity: 97% (SDS-PAGE and HPLC) Endotoxin: 1EU/ug (LAL) Biological Activity: Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 8x10e4 IU/mg. Amino Acid Sequence: IAGPEWLLDR PSVNNSQLVV SVAGTVEGTN QDISLKFFEI DLTSRPAHGG KTEQGLSPKS KLFATDSGAM PHKLEKADLL KAIQEQLIAN VHSNDDYFEV IDFASDATIT DRNGKVYFAD KDGSVTLPIQ PVQEFLLKGH VRVRPYKEKP VQNQAKSVDV EYTVQFTPLN PDDDFRPALK DTKLLKTLAI GDTITSQELL AQAQSILNKN HPGYTIYERD SSIVTHDNDI FRTILPMDQE FTYHVKNREQ AYRINKKSGL NEEINNTDLI SEKYYVLKKG EKPYDPFDRS HLKLFTIKYV DVNTNELLKS EQLLTASERN LDFRDLYDPR DKAKLLYNNL DAFGIMDYTL TGKVEDNHDD TNRIITVYMG KRPEGENASY HLAYDKDRYT EEEREVYSYL RYTGTPIPDN PNDK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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