Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). {ECO:0000269|PubMed:19118384, ECO:0000269|PubMed:7533181}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 ug/ml in the presence of 2ng/ml of rHuFas Ligand. Sequence: RLSSKSVNAQ VTDINSKGLE LRKTVTTVET QNLEGLHHDG QFCHKPCPPG ERKARDCTVN GDEPDCVPCQ EGKEYTDKAH FSSKCRRCRL CDEGHGLEVE INCTRTQNTK CRCKPNFFCN STVCEHCDPC TKCEHGIIKE CTLTSNTKCK EEGSRSN Molecular Weight: 17.6kD PubMed ID: 1713127, 1375228, 7575433, 7533181, 8598453, 8648105, 17336828, 14702039, 15164054, 15489334, 7538908, 10542291, 10535980, 14759258, 15465831, 18691976, 18669648, 19159218, 21109225, 20068231, 21269460, 22171320, 24275569, 8967952, 19118384, 7540117, 8929361, 9028321, 9028957, 9322534, 9787134, 9821419, 10090885, 10515860, 10e340403, 9927496, 10620127, 11418480, 20935634 Gene Name: FAS,APT1,FAS1,TNFRSF6 Swiss Prot: P25445 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
