Product Description
The carbohydrate binding site recognizes (beta-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to Nacetyllactosamine and oligomers containing repeating -acetyllactosamine sequences. Abranched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (beta-1,6) and (beta-1,2) was reported to be the most potent inhibitor of agglutination. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Sugar Specificity: [GlcNAc]1-3, N-Acetylglucosamine Inhibiting/Eluting Sugar: Chitin Hydrolysate Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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