Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors. Recombinant murine IFNg is a 15.6kD protein containing 134 amino acid residues. Biological Activity: Determined by its ability to inhibit the proliferation of mouse WEHI-279 cells. ED50 is 0.2ng/ml, corresponding to a specific activity of 5 x 10e6units/mg. Amino Acid Sequence: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA, HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
15.6
Purity:
98% by SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug).
Form:
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0, 25mM sodium chloride. Reconstitute in ddH2O to 1mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA).
* VAT and and shipping costs not included. Errors and price changes excepted
