Product Description
Encoded by the ACP5 gene, Tartrate Resistant Acid Phosphatase (TRACP or TRAP) is also known as Purple Acid Phosphatase (PAP) or Acid Phosphatase 5 (ACP5). The deuced amino acid sequence of human ACP5 (Accession NP_001602) predicts a signal peptide (aa1-21) and a mature chain (aa22-325). rhACP5 consists of aa22- 320, without the last 5 residues, RRARP. ACP5 is expressed at high levels by osteoclasts, macrophages and dendritic cells. Two forms, 5a and 5b, circulating in human blood, are derived from different cell types and have different functions. Derived from macrophages and dendritic cells, 5a is a marker of inflammatory conditions. Derived from osteoclasts, 5b is a marker of bone resorption. Compared to 5a, 5b does not contain sialic acid residues, has a higher specific activity and pH optimum, and may be processed into a disulfide-linked dimer. Murine myeloma cell line, NS0-derived Ala22-Pro320 with a C-terminal 6-His tag. Accession: NP_001602 Molecular Mass: The 305 amino acid residue corresponding to recombinat human TRAP 5 predicts a molecular mass of 34kD and migrates as a triplet with apparent molecular masses between 36-40kD in SDS-PAGE under reducing conditions. Activity: 65,000pmoles/min/ug. Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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