Product Description
Control Peptide for T8269-73. Minor disruption of the GI surface layer of the cells occurs frequently, such that maintaining epithelial integrity is of crucial importance. Normal epithelial repair requires restitution and regeneration. During restitution, within minutes after injury, epithelial cells spread and migrate across the basement membrane to re-establish surface cell continuity. Trefoil factor family (TFF) proteins play an important role in restitution by influencing the migration of cells without promotion of proliferation or tumorigenesis. Regeneration occurs later on and involves proliferation and differentiation of epithelial cells and restoration of specialized elements. Three mouse TFFs have been identified to date: TFF1 (formerly pS2) and TFF3 (formerly hITF), with one trefoil domain each, and TFF2 (formerly hSP), with two trefoil domains. TFF1: rat: 81aa, human: 84aa, mouse: 87aa - 9.67kD, Mouse chromosome: 17A3.3 . Expressed in a number of carcinomas including breast (50%), pancreas (70-75%), stomach (50-55%), and large bowel (58%). Synthetic peptide corresponding to 15aa near the C-Terminal of mouse TFF1 (Accession Q08423) Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 50-100ng control peptide/well Antibody Blocking: 5-10ug/1ug T8269-73 or 1ul antiserum. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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