Control Peptide for T8269-75A. Minor disruption of the GI surface layer of the cells occurs frequently, such that maintaining epithelial integrity is of crucial importance. Normal epithelial repair requires restitution and regeneration. During restitution, within minutes after injury, epithelial cells spread and migrate across the basement membrane to re-establish surface cell continuity. Trefoil factor family (TFF) proteins play an important role in restitution by influencing the migration of cells without promotion of proliferation or tumorigenesis. Regeneration occurs later on and involves proliferation and differentiation of epithelial cells and restoration of specialized elements. Three mouse TFFs have been identified to date: TFF1 (formerly pS2) and TFF3 (formerly hITF), with one trefoil domain each, and TFF2 (formerly hSP), with two trefoil domains. Tff3: human: 101, rat: 81, mouse: 81aa - 8.8kD, Human chromosome: 17A3.3. TFF3 is expressed in intestinal goblet cells, gland acini, distal ducts of Brunners glands, human uterus, normal breast, respiratory epithelium and some regions of the hypothalamus. Source: Synthetic peptide corresponding to 16aa of human TFF3 (Accession Q96NX0), C-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
