The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Biotinylated WGA has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide. Source: Wheat germ Blood Specificity: A, B, O Carbohydrate Specificity: N-Acetylglucosamine Inhibitory Carbohydrate: Chitin Hydrolysate or N-Acetylglucosamine Divalent Ions: None Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology Recommended Dilutions: For most applications we recommend a freshly prepared working solution of 5-20ug/ml in the above buffer. Activity: <4mg/ml will agglutinate human type O erythrocytes. <1ug/ml will agglutinate neuraminidase treated erythrocytes. Quality Control: SDS-PAGE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Molecular Weight:
36
Form:
Supplied as a liquid in PBS, pH 7.3. Labeled with Biotin.
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