The biotinylated horse anti-goat IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
Suitable for use with goat, sheep, and bovine IgG primary antibodies
Recognizes both heavy and light chains (H+L)
Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albuminor non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. NOTE: This product is raised in horse and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of goat IgG which is raised in rabbit.
Target Species
Goat
Conjugate
Biotinylated
Host Species
Horse
Format
Concentrate
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