ChIP-Exo-Seq

Validated Antibodies from Atlas Antibodies

ChIP-ExoSeq technology improves upon the ChIP and ChIP-Seq methods by offering greater resolution and specificity. However, many commercially available antibodies perform poorly.

Our partner Atlas Antibodies sets a higher benchmark for antibody performance in ChIP assays. With a validation success rate of 50%, more than double the industry average, Atlas Antibodies' ChIP-certified HPA polyclonals demonstrate exceptional specificity and reproducibility in ChIP-Exo-Seq assays. This ensures sharper, more accurate peak detection and the identification of exact protein-DNA binding sites.

 

ChIP-Exo-Seq: Next-Generation ChIP Assays

ChIP-Exo-Seq stands for Chromatin Immunoprecipitation (ChIP) coupled with exonuclease digestion (exo) and high-throughput (NGS) sequencing (seq). This technology is a further refinement of the ChIP and ChIP-Seq methods, providing superior resolution and specificity by incorporating exonuclease digestion to pinpoint protein binding sites to the exact base pair (1 bp).

The ChIP-Exo-Seq validation gives a detailed view of protein-DNA interactions, allowing for precise insights at the single-base nucleotide level. ChIP-Exo-Seq offers higher resolution and strand specificity compared to ChIP and ChIP-Seq, while reducing background noise for more accurate mapping of protein-DNA interactions.

Advantages of ChIP-Exo-Seq Over Traditional ChIP and CUT&RUN/TAG Assays

• Higher Resolution: ChIP-Exo-Seq achieves near single-base resolution of protein-DNA interactions, whereas other assays typically identifies broader regions.
• Reduced Noise: Exonuclease trimming eliminates background DNA that isn’t directly protected by the protein, reducing false positives.
• Precise Binding Site Localization: the sharp peaks from ChIP-Exo-Seq provide more accurate mapping of transcription factor binding sites or other protein-DNA interactions.
• Greater reliability especially for “difficult” targets.

 

The success of ChIP assays depends on the quality of the primary antibodies used for immunoprecipitation. ChIP relies on antibodies to selectively enrich DNA fragments bound by a specific protein (e.g., transcription factor, histone modification). Primary antibodies must bind with high specificity and affinity to avoid pulling down unrelated proteins or DNA, which can cause false positives or background noise. Poorly validated antibodies can lead to non-reproducible results, compromising downstream analysis like motif discovery or peak calling.

 

High-Quality Antibodies for ChIP Applications from Atlas Antibodies

Atlas Antibodies is at the forefront of chromatin research, offering a comprehensive portfolio of high-quality, rigorously validated antibodies tailored for ChIP-Exo-Seq applications. As epigenetic research demands ever-greater precision, the reliability of antibodies used in ChIP-based assays becomes a critical determinant of data accuracy. To address this, Atlas Antibodies has developed and validated a range of antibodies specifically optimized for high-resolution chromatin mapping, ensuring robust and reproducible results.

Through ongoing collaboration with Dr. Frank Pugh, a pioneer in ChIP technology, Atlas Antibodies plays a pivotal role in advancing ChIP-Exo-Seq methodologies. The partnership with Dr. Pugh has enabled Atlas Antibodies to optimize antibody specificity and performance specifically for ChIP-Exo-Seq applications. This collaborative effort has resulted in the validation of a wide catalog of antibodies with that enhanced resolution and reproducibility, addressing critical challenges in chromatin immunoprecipitation techniques.

Those antibodies are labelled as "ChIP certified" in our webshop.

>> Find all ChIP certified Antibodies from Atlas Antibodies

 

 

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