CUT&Tag-IT Assay Kits

Rapid and robust genome-wide analysis of histone marks at lower sequencing depths

Active Motif is the industry leader in developing and delivering innovative tools to enable epigenetics and gene regulation research. With CUT&Tag-IT® they developed a method for rapid and robust genome-wide analysis of histone marks without the need of NGS library preps.

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CUT&Tag (Cleavage Under Targets and Tagmentation) utilizes a protein A (pA) Tn5 chimera (pA-Tn5) and a mouse or rabbit primary antibody to a histone or histone mark of interest to tether the pA-Tn5. This directs the Tn5 activity to tagment the DNA around the target of interest genome-wide, without any NGS library preparation steps, to reveal the genetic sequences of those sites of interest. CUT&Tag requires much less input material and sequencing read depths than ChIP-Seq, and can be perfomed in 1-2 days.

 

Available CUT&Tag Assay Kits:

CUT&Tag-IT® Express Assay Kit

Less variable and higher-throughput genome-wide profiling of histone marks.

Product Description	Format	Art. N°
CUT&Tag-IT® Express Assay Kit	16 rxns	ACM-53175
	48 rxns	ACM-53177

 

CUT&Tag-IT® Assay Kit - Cells

Profile histone marks with rabbit or mouse antibodies from as few as 5,000 cells.

Product Description	Format	Art. N°
CUT&Tag-IT® Assay Kit, Anti-Rabbit	16 rxns	ACM-53160
	3 x 16 rxns	ACM-53171
CUT&Tag-IT® Assay Kit, Anti-Mouse	16 rxns	ACM-53165
	3 x 16 rxns	ACM-53172
CUT&Tag-IT® Core Assay Kit	16 rxns	ACM-53176

 

CUT&Tag-IT® Assay Kit – Tissue

Profile histone marks with rabbit or mouse antibodies from 0.5 to 10 mg of tissue.

Product Description	Format	Art. N°
CUT&Tag-IT® Assay Kit - Tissue	16 rxns	ACM-53170

 

CUT&Tag-IT® Spike-In Control

Compare between CUT&Tag assay datasets with confidence.

Product Description	Format	Art. N°
CUT&Tag-IT® Spike-In Control, Anti-Rabbit	16 rxns	ACM-53168
CUT&Tag-IT® Spike-In Control, Anti-Mouse	16 rxns	ACM-53173

 

CUT&Tag-IT® R-loop Assay Kit

Genome-wide profiling of DNA-RNA Hybrid R-loops.

Product Description	Format	Art. N°
CUT&Tag-IT® R-loop Assay Kit	16 rxns	ACM-53167

 

CUT&Tag-IT® Spike-In Control, R-loop

Analyze CUT&Tag-IT R-loop Assay datasets confidently to detect true biological differences.

Product Description	Format	Art. N°
CUT&Tag-IT® Spike-In Control, R-loop	16 rxns	ACM-53174

 

Accessory Product:

Tissue Prep for NGS Assay

Tissue sample preparation for CUT&RUN, CUT&Tag, or CUT&Tag R-loop Assays.

Product Description	Format	Art. N°
Tissue Prep for NGS Assays Kit	24 rxns	ACM-53185