Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
PLoS One, 2017
Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
MHC tetramers bind stably to cognate T-cell receptors and consequently can be used as detection tools. Our partner MBL offers a comprehensive range of MHC tetramers and MHC multimers. Phycoerythrin (PE), allophycocyanin (APC), or Brilliant Violet™ 421 (BV421) fluorochromes are available for detection of antigen-specific T cells by flow cytometry or fluorescence microscopy.