Goat IgG anti-Human IgG (F(ab)2)-HRPO, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Human IgG (F(ab)2)-HRPO, MinX none
• Artikelnummer: ROC-209-1304
• Hersteller Artikelnummer: 209-1304
• Alternativnummer: ROC-209-1304
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Anti-Human IgG F(ab)2 was produced by repeated immunization with human IgG F(ab)2 fragment in goat.
• Konjugation: HRPO
• Alternative Synonym: Human IgG F(ab)2 Antibody, Gt-a-Human F(ab)2 Peroxidase conjugated, Human F(ab)2 Peroxidase Antibody in goat, Goat F(ab)2 peroxidase conjugated Secondary Antibody.
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 10.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Anwendungsbeschreibung: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to

Bilder

Whole sperm ELISA to assess the binding potency of the indicated Abs to human sperm. The serial dilution of mAbs in 1% milk was added to the microwells and incubated overnight at 4C. Motavizumab (anti-RSV IgG1) was used as the isotype control. After primary incubation, the plates were washed three times using 1X PBS. Then, the secondary Ab, goat anti-human IgG F(ab)2Ab HRP-conjugated (1:10,000 dilutions in 1% milk, p/n 209-1304) was added to the wells and incubated for 1 hr at room temperature. Data represent 3 independent experiments with 3 unique semen donors. Each experiment was performed in triplicates and averaged. Lines indicate arithmetic mean values and standard deviation. Figure S4. PMID: 32937206.

ELISA results using Goat Anti-Human IgG F(ab)2 Antibody Peroxidase Conjugated.High dose IVIg reduces the circulation kinetics of bispecific Ab by 3-fold.A) The serum circulation profile of bispecific Fab-IgG1(30 µg) in athymic nude mice (n = 8 total mice, 4 mice per time point).B) The serum circulation profile of bispecific Fab-IgG1(30 µg) in the presence of high dose IVIg (30 mg) in athymic nude mice (n = 8 total mice, 4 mice per time point). The solid line for both figures represents the predicted fit for a two-compartment model used to calculate the elimination half-life (t1/2), volume of distribution (VD), and clearance (CL). Figure 3. PMID: 31394261.